Padmini Jayaraman Email & Phone Number

Padmini Jayaraman Skills

• Cancer Research
• Business Development
• Drug Development
• Oncology
• Cell Culture
• Molecular Biology
• Flow Cytometry
• Biochemistry
• Western Blotting
• Cell Biology
• PCR
• ELISA
• Tissue Culture
• Life Sciences
• Animal Models
• Transfection
• Molecular Cloning
• Cell
• Immunology
• RT-PCR
• • Tissue culture techniques- Maintaining mammalian cell line, generation of bone marrow derived macrophages/dendritic cells etc. • Immunological techniques- Generation of hybridoma, generation of bone marrow chimera and adoptive transfer in mice, multi-color and multi-parameter flow cytometry, ELISPOT, cytotoxic T cell assay, ELISA, Multiplex cytokine assay, cytotoxicity assay, lymphocyte proliferation assay, estimation of various cytokines, MDSC generation and suppression assay, phagocytosis assay, western immunoblotting. Generation of 3D spheroids. • Cell biology technique- Con-focal microscopy, transfection, retro-viral transduction assay, and Fluorescence microscopy. • Hands-on experience in micro, molecular and cellular biological methods, including: DNA/RNA extraction and analysis, DNA sequencing, cloning, Real-time quantitative PCR, promoter assay etc. . • Microbiology techniques –microbial cultures, colony forming units (CFU) enumeration etc. • Skilled in mouse husbandry and manipulations involving intravenous / subcutaneous /intradermal/intraperitoneal injections, dissections of lympnodes/spleens/lungs/thymus and tumor and adoptive transfer experiments. Experienced in bone marrow and fetal liver transplantations. • Trained to work with human patient samples, derive blood and obtain polymorphonuclear cells and use them in experiments. • Strong critical thinking/analysis capability; excellent written and oral communications abilities; well-developed platform skills, particularly in presenting complex data to both scientists and non-scientists; comfort level with multi-tasking. • Adept at training /teaching research methodologies and science to undergraduate, graduate, medical students and medical residents.
• Multi-color Flow Cytometry
• Small Animal Models
• Cell imaging
• IVIS imaging
• Research
• Laboratory Skills
• Microsoft Office
• Project Management
• Genetics
• Clinical Research
• Leadership
• Research and Development (R&D)