Matteo Bertolotti Email & Phone Number

Matteo Bertolotti Skills

• biochemistry
• biology
• cosmetics
• Protein Chemistry
• ELISA
• In situ Hybridization
• Bacterial Cell Culture
• Bacterial Transformation
• Vector Cloning
• In vitro protein phosphorylation
• Mass and Mass/Mass spectometry (MALDI-ToF-ToF)
• HPLC/MS analysis (ESI-Quadrupole)
• Protein protein interaction identification by Protein Arrays
• Heterologous proteins expression and production in E.coli and Insect cells.
• Protein purification by affinity chromatography
• Protein enzymatic digestion and limited proteolysis
• N-terminal sequencing (Procise cLC)
• GST Pull-down
• Gel Kinase assay
• RNA extraction from cells and tissues
• RT qPCR with ABI Prism 7900HT and Biorad IQ5
• Liquid handler programming
• NADH coupled atpase assay
• ATPase activity assay (Promega ADPGLO)
• Buffer optimization for enzymatic reactions
• Km and IC50 calculation
• Protein lysates printing on slice (Biorad Calligrapher)
• RPPA Fast green dying and Immunodecoration
• Microsomial enriched fractions preparation from insect cells
• Bacterical and yeast coltures
• Growth kinetics and FACS analysis of yeast cells
• Human Primary Cell lines cultures
• Transient transfections and RNA interference
• yeast cells culture
• W/O Emulsion
• O/W Emulsion
• Hydrogel
• Toiletries