Kyle Edewaard Email & Phone Number

Kyle Edewaard Skills

• Cell culture of primary and established cell lines
• Isolation of primary mouse brain endothelial cells, astrocytes, microglia, neu
• Splenic T cell isolation through negative selection
• T cell activation
• Scratch wound growth assay
• Immunocytochemistry
• Flow Cytometry (FACSCanto)
• Cellular Adherence Assays
• Evaluate transendothelial electrical resistance (TEER)
• Maintenance of breeders, progeny, and associated records
• Mouse Husbandry
• Mouse Tagging and Tailing
• Genotyping
• Cell Staining / Dye titration
• DNA isolation, quantification, and qualification
• Restriction enzyme digestion
• Antibody titration
• Expression cloning: giardial transformation, selection, and screening
• 1D agarose and acrylamide gel electrophoresis
• Primer design
• Polymerase chain reaction
• Cytokine Analysis (ELISA)
• Plaque Assay: viral titer, load, or replication determination
• Large scale viral stock preperation
• In vivo (mouse) viral infection (intraperitoneal)
• Sample harvest (mouse) for viral titer, pathogenesis, and immunology: brain, spl
• BCA protein quantification
• 1D gel electrophoresis
• Western blot
• Gelatin zymography
• Mac and PC operating systems
• Graphpad Prism
• Apple Aperature
• Excel, Word, Powerpoint
• FlowJo
• Quantity One: analysis of protein and DNA gels
• Metamorph microscopy imaging and metamorphic quantification of histopathology ph
• Assay Development
• Laboratory
• ELISA
• Western Blotting
• Data Analysis
• Science
• PCR
• Research
• Tissue Culture
• Genetics
• Molecular Biology
• Biochemistry
• Cell
• Sample Harvest Mouse For Viral Titer
• Screening
• Viral
• Mac And Pc Operating
• Selection
• Dye
• Immunology
• Metamorph Microscopy
• Cytokine Analysis
• Bca Protein
• Evaluate Transendothelial Electrical Resistance Teer
• Quantification
• Splenic T Cell
• Neu
• Plaque Assay
• Flow Cytometry Facscanto
• Polymerase Chain
• PowerPoint
• Dye Titration
• Qualification
• Expression Cloning
• Load
• Scratch Wound Growth
• Analysis Of Protein And Dna Gels
• Viral Titer
• Mouse Tagging
• Word
• Cytokine Analysis Elisa
• Analysis
• Pathogenesis
• Quantity One
• In Vivo Mouse Viral
• Evaluate
• Brain
• Or Replication Determination
• Progeny
• Restriction Enzyme
• Spl
• Giardial Transformation
• Sample Harvest Mouse
• Flow Cytometry
• 1D Agarose
• Isolation Of Primary Mouse Brain Endothelial Cells
• Microglia
• Maintenance Of Breeders
• Large Scale Viral Stock
• Astrocytes
• Excel
• Isolation Of Primary
• Cell Staining
• Associated Records
• Cellular Adherence
• In Vivo Mouse Viral Infection Intraperitoneal
• Dna Isolation
• Cell Culture Of Primary
• Sample Harvest for Viral Titer
• Evaluate Transendothelial Electrical Resistance
• Sample Harvest
• In Vivo Viral
• In Vivo Viral Infection