John Townsend Email

John Townsend Skills

• Aseptic Processing
• Assays, harvest, centrifugation, automation
• Biological safety basics and aseptic techniques
• Bioreactor Design Principles
• Document Management
• Equipment cleaning, sterilization, disposables, corrective actions
• External Audits
• Familiar with CIP (Clean in Place) and SIP (Steam in Place)
• Fermentation parameters, heat, agitation, aeration, operating cost
• GDP
• GFRC (Gas Flow Ratio Controller)
• GMP
• Genetic engineering to alter microbes to produce recombinant peptides & proteins
• Good Laboratory Practice (GLP)
• Inactivation system, area clearance, auditing, changeover
• Inoculum preparation and storage of microbial cultures in cell banks
• Internal Audits
• Laboratory Information Management System (LIMS)
• Large-scale safety, cleanroom operations, cGMP documentation
• Medical Devices
• Methods of controlling critical process parameters
• Microbial death kinetics, heat transfer and process design
• Microbial identification by morphology, physiology and genome sequencing.
• Microbial production of alcohols & antibiotics
• Microsoft Excel
• Microsoft Office
• Optimizing bioreactor performance, Critical Control Parameters, Process Control
• Oxygen mass transfer coefficient: kLa & effects of agitation and aeration
• PID (Proportional, Integral, Derivative)
• Pharmaceutics
• Preparing liquid culture media and solid agar media
• Production of enzymes and antibody fragments in bacterial expression systems.
• Production of recombinant proteins in microbial hosts using 2L fermentors.
• Production of recombinant proteins in microbial hosts using shake flasks.
• Quality systems, failure investigations, components, water systems
• Quantification of cell growth, growth yields and batch growth kinetics
• SAP
• Scheduling, process variability, automation, Process Analytical Technology (PAT)
• Seed banking, cell growth, product expression, and change control
• Seed growth checks, probe calibration, transfers, growth rates
• Supplier Audits
• Understanding rationale for strain selection, development, process optimization
• Vaccines
• cell counting/freezing of E. coli and CHO cells,preservation of S. cerevisiae