Dhanesh B Email

Dhanesh B Skills

• SDS-PAGE and Western Blotting
• Recombinant Protein production and purification
• Protein isolation and quantification
• 2Photon Imaging
• STED Imaging
• Live Cell Imaging
• Confocal Microscopy
• Fluorescence Microscopy
• Cryosectioning
• FACS analysis
• Immunohistochemistry
• CRISPR based bioenginnering
• Development of CRISPR based cell-specific genome editing tools
• Infusion Cloning
• Molecular Cloning
• DNA sequencing- Sangers and NGS
• Site Directed Mutagenesis
• RNA Isolation
• Genomic and Plasmid DNA Isolation
• Agarose Gel Electrophoresis
• Bacterial Transformation
• Competent cell preparation
• Cranial window surgery for 2P imaging
• Mouse surgery to implant head mount EEG electrodes and EEG recording
• Stereotaxic injection into mouse cortex
• Drugs/BrdU/Biotin administration (IP/Subcutaneous/intramuscular/oral)
• Intra Cardial Perfusion of mice
• Development of transgenic mice (From chimera to pure line)
• Dissection of embryonic and adult brain
• Intracortical and retro-orbital injection of AAV
• Postnatal Astrocyte Labeling by Electroporation (PALE)
• Ex vivo electroporation of embryonic brain and retina
• In utero electroporation of embryonic brain and retina
• Adeno-associated virus(AAV) production, and purification
• Lentivirus production
• Luciferase Assay
• Transfection (Lipofectamine, PEI, Biolistics and Electroporation methods)
• Embryonic Stem (ES) Cell culture and ES-NP generation (Mouse D3-ES Cells)
• Mammalian cell lines culture (HEK293T, IMR-32, U87MG, 3T3, VM etc)
• Primary cortical(mixed), neuron and astrocyte culture
• Cortical Slice Culture
• Reverse Transcription Polymerase Chain Reaction (RT-PCR)
• Real-Time Polymerase Chain Reaction (qPCR)
• Polymerase Chain Reaction (PCR)
• in Situ Hybridization
• Genome Editing
• Immunocytochemistry
• Research and Development (R&D)