Darshan Pathak Email & Phone Number

Darshan Pathak Skills

• Antibody Purification and Labeling
• Bioinformatics
• Biology
• Business Innovation
• Cell Biology
• Cell Fractionation
• Creativity and Innovation
• DNA Sequencing
• Directed Evolution
• Fluorescence Microscopy
• Fluorescent and phase contrast microscopy
• Genetic Engineering
• Genetics
• HPLC
• Immunofluorescence
• Immunology
• Microbiology
• Microscopy
• Molecular Biology
• Molecular Cloning
• PCR
• PCR and Electrophoresis
• Protein Expression
• Protein Expression and Purification, Cell-free Protein Synthesis, RNA Isolation, Enzyme Kinetics, CO-IP
• Protein Labeling
• Protein Purification
• Protein localization and dynamics
• Protein-protein Interactions
• Synthetic Biology
• TECHNICAL SKILLS Organism: C. crescentus, B. thetaiotaomicron, B. fragilis, M. xanthus, M. fulvus E. coli Molecular Cloning: Genetic design and implementation, Gibson Assembly, Golder Gate Assembly, Type-II restriction Analytical Tools: Luminometry, ELISA, Western Blot, RT-PCR, HPLC, Microscopy (Florescence, EM) Biochemistry: Protein Expression and Purification, Cell-free Protein Synthesis, RNA Isolation, Enzyme Kinetics, CO-IP Microbial engineering: Implementation of knock in knock out method, Pathway Design, Conditional Expression, Mutagenesis Microbiology: BSL-2, Competition Assays, MIC Determination, Bac 2 Hybrid, Conjugation, Transduction, Transformation
• Time-lapse Microscopy
• Western Blotting
• qPCR