Chris Bohl Email & Phone Number

Chris Bohl Skills

• • Extensive experience with mammalian cell culture (11 years)
• • BSL-2 and BSL-3 (11 years)
• • Transient transfection of several mammalian cell lines
• • Virus production, purification, titration, and infection
• • siRNA and shRNA knockdown
• • Cloning stable cell lines, isolation, and characterization
• • Protein expression and purification from E. coli and mammalian cell lines
• • Immunofluorescence/Confocal Microscopy
• • SDS-PAGE and immunoblotting (HRP and Immunofluorescence)
• • ELISA
• • Cloning
• • plasmid DNA preps (Mini and Maxi)
• • Isolation and quantification of genomic DNA from mammalian cells
• • PCR and semi-quantitative RT-PCR
• • Northern and Southern blotting
• • Metabolic labeling, phosphor imaging, and quantification
• • Ultracentrifugation and isopycnic centrifugation with sucrose gradients
• • Flow cytometry
• Western Blotting
• Molecular Biology
• Enzyme Assays
• Metabolism
• Cell Biology
• ELISA
• Viruses
• Transfection
• Metabolic Labeling
• Plasmid Dna Preps Mini And Maxi
• Isolation
• Wood
• Pcr And Semi Quantitative Rt Pcr
• PCR
• DNA
• Virology
• Cell Culture
• Healthcare
• Ultracentrifugation And Isopycnic Centrifugation With Sucrose Gradients
• Virus Production
• Transient Transfection Of Several Mammalian Cell Lines
• Supervision
• Purification
• Characterization
• Isolation And Quantification Of Genomic Dna From Mammalian Cells
• Titration
• Quantification
• Microsoft Office
• Immunofluorescence/Confocal Microscopy
• Microsoft Excel
• Phosphor Imaging
• Transient Transfection
• Customer Service
• Sds Page And Immunoblotting Hrp And Immunofluorescence
• Extensive Experience With Mammalian Cell Culture 11 Years
• Replication
• Analytical Ultracentrifugation
• Immunoblotting
• Molecular Cloning
• Protein Expression
• Cloning
• Northern And Southern Blotting
• Infection
• Genetics
• Research
• Biochemistry
• Bsl 2 And Bsl 3 11 Years
• Flow Cytometry
• Centrifugation
• Cloning Stable Cell Lines
• Sirna And Shrna Knockdown
• Tissue Culture
• Microscopy
• Protein Expression And Purification From E. Coli And Mammalian Cell Lines
• Teaching
• Data Analysis
• Extensive Experience With Mammalian Cell Culture
• Bsl 2 and Bsl 3
• Sds Page and Immunoblotting
• Plasmid Dna Preps